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New primers for molecular sex identification of waders

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Pages
147 – 151

Published
1 August 17

Authors
Marco van der Velde, Oliver Haddrath, Yvonne I. Verkuil, Allan J. Baker, Theunis Piersma

DOI
10.18194/ws.00069

Correspondence
Marco van der Velde
m.van.der.velde@rug.nl
Behavioural and Physiological Ecology, Groningen Institute for Evolutionary Life Sciences, University of Groningen, P.O. Box 11103, 9700CC Groningen, Thee Netherlands

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Determining the sex of individuals through molecular methods is an increasingly important research tool, particularly for studies of sexually monomorphic species. However, currently available primer sets to molecularly sex birds are known to cause difficulties in sexing Red Knots Calidris canutus and several other waders, due to amplification failure of, and polymorphisms on, the CHD-Z gene. To counter these problems, we developed a new primer set, 2602F/2669R, and show that these primers can be used to reliably sex Red Knots and eight other wader species in four genera. We expect these primers to be useful in a wide range of wader and possibly non-wader species.